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Learn about fidelity of a DNA polymerase, methods for measuring enzyme fidelity, and benefits of using a high-fidelity enzyme in your PCR. | Invitrogen
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TAQ Polymerase is highly stable at high temperatures, specifically at the DNA denaturation temperature (~94-98°C). This stability is crucial for the PCR process, which requires repeated heating and cooling cycles. In contrast, many other DNA polymerases would denature or become inactivated at high temperatures. 5' to 3' Polymerase Activity: Taq polymerase has a 5' to 3' DNA polymerase activity, meaning it can synthesize new DNA strands by adding nucleotides in the 5' to 3' direction. This is the
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